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Sequence determination of the capsid protein gene and flanking regions of tobacco etch virus: Evidence for synthesis and processing of a polyprotein in potyvirus genome expression*

机译:烟草蚀纹病毒衣壳蛋白基因和侧翼区域的序列测定:在杯状病毒基因组表达中合成和加工多蛋白的证据*

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摘要

The nucleotide sequence of the 3′-terminal portion of the tobacco etch virus (TEV) genome was determined. The 2324-nucleotide sequence represented approximately one-fourth of the TEV genome and included the capsid protein gene and flanking regions. An open reading frame of 2135 nucleotides and an untranslated region of 189 nucleotides adjacent to a polyadenylate tract were identified. The sequence began within an open reading frame, indicating that the initiation codon was upstream of the available sequence data. The sequence of the 20 NH2-terminal amino acids of the TEV capsid protein was established chemically. An identical amino acid sequence, predicted from the nucleotide sequence, was located, commencing at amino acid - 263. These data indicated that maturation of the capsid protein required a post-translational cleavage of a larger protein precursor, with a probable cleavage site between the amino acids glutamine and glycine.
机译:确定了烟草蚀刻病毒(TEV)基因组3'-末端部分的核苷酸序列。 2324个核苷酸的序列约占TEV基因组的四分之一,并包括衣壳蛋白基因和侧翼区域。鉴定出2135个核苷酸的开放阅读框和189个核苷酸的非翻译区与聚腺苷酸束相邻。该序列在开放阅读框内开始,表明起始密码子在可用序列数据的上游。通过化学方法确定了TEV衣壳蛋白的20个NH 2-末端氨基酸的序列。根据核苷酸序列预测,找到了一个相同的氨基酸序列,起始于第263位氨基酸。这些数据表明,衣壳蛋白的成熟需要翻译后的较大蛋白前体裂解,而在前者之间可能存在裂解位点。氨基酸谷氨酰胺和甘氨酸。

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